On Earth-65, Spider-Woman Gwen Stacy finds herself growing distant from her friends and estranged from her father, George Stacy. George, unaware of the full truth, is hunting Spider-Woman, mistakenly believing she killed Peter Parker. In reality, Peter's death was a tragic accident after he transformed into the Lizard. Gwen encounters a variant of the Vulture from a Renaissance-themed parallel universe. To help neutralize this threat, Miguel O’Hara and Jess Drew arrive through a dimensional portal. Cornered by George, Gwen reveals her true identity to him. Despite this, he attempts to arrest her, leading Gwen to ultimately leave with Jess and Miguel.